Table 1.
Identity of selected proteins extracted from rice leaves, which differ significantly in abundance between different leaf ages and Mn treatments
| Protein no.* | Fold induction/reduction† | Coverage (%)‡ | Accession no.§ | Identity¶ |
|---|---|---|---|---|
| Fold induction/reduction in the comaprison of optimum and excess Mn- (50 µm) treated old (fourth) leaves | ||||
| 2 | 0·44 | 28·40 | BAD16832 | Chloroplast translational elongation factor Tu |
| 7 | Only present at 1 µm Mn | 29·90 | NP_001048798 | 50S ribosomal protein L11 |
| Fold induction/reduction in the comaprison of optimum and excess Mn (50 µm) treated young (sixth) leaves | ||||
| 5 | 2·25 | 34·40 | BAD16934 | Putative inorganic pyrophosphatase |
| 8 | 2·16 | 67·70 | NP_001066998 | Probenazole-inducible protein PBZ1 |
| 9 | 3·40 | 18·00 | NP_001054704 | Universal stress protein (Usp) family protein |
| Fold induction/reduction in the comparison of old leaves (fourth) and young (sixth) leaves of plants treated with optimum Mn supply continiously | ||||
| 1 | 0·49 | 48·40 | Q42971 | Enolase (2-phosphoglycerate dehydratase) |
| 4 | 2·21 | 33·90 | NP_001043134 | Oxygen-evolving enhancer protein 1, chloroplast precursor |
| 6 | 2·07 | 40·10 | NP_001063793 | Ribonuclease T2 family protein |
| Fold induction/reduction in the comparison of old leaves (fourth) and young (sixth) leaves of plants treated with excess Mn supply for 4 d | ||||
| 3 | 0·47 | 43·70 | NP_001047479 | Adenosine 5′- phosphotransferase 2 |
| 7 | Only present in sixth leaf | 36·20 | NP_001048798 | 50S ribosomal protein L11 |
| 8 | 2·64 | 66·50 | NP_001066998 | Probenazole-inducible protein PBZ1 |
| 10 | 2·51 | 14·20 | NP_001058899 | 40S ribosomal protein S12 |
| 11 | 0·47 | 33·10 | A2YQD9 | Ferredoxin-1, chloroplast precursor (Ferredoxin I) (anti-disease protein 1) |
| 12 | 2·31 | 19·30 | P56724 | 60S acidic ribosomal protein P3 (P1/P2-like) |
| 13 | 3·06 | 14·80 | NP_001043653 | Ribosomal protein L31 family protein |
* Numbers of spots corresponding to Fig. 9.
† Regulation of spots between compared populations of gels based on mean relative spot volume.
‡ Sequence coverage of the identified proteins with the corresponding NCBI database hit.
§ Accession number of the identified protein in the NCBI database.
¶ Identity of the protein.