Table 1.
List of genes and specific primer pairs used for quantitative gene expression analysis
| Candidate gene | Primer pairs (5′ → 3′)* | Amplicon size (bp) | GenBank accession no. |
|---|---|---|---|
| PFK (phosphofructokinase) | (+) ACCCTTGCAAGTCGAGATGT | 171 | FE688067 |
| (−) CTGCACACTCTCGGAAACAA | |||
| PEPC (phosphoenolpyruvate carboxylase) | (+) TGGCTCCTTCCAAAGTGAGT | 100 | AF288382 |
| (−) TCCTCCCCGTGTAAATTCTG | |||
| CS (citrate synthase) | (+) CGACGGATATTCAAGGATGG | 142 | FE693132 |
| (−)CGTGATCACTGTGGATGGAA | |||
| Aco (aconitase) | (+) TCCAGTGTGTTGCCTGACAT | 116 | CV536664 |
| (−) GACTTGGGGTCCCATGAGTA | |||
| ICDH (isocitrate dehydrogenase) | (+) GCTCATTTTGCCTTTCCTG | 165 | SSH library |
| (−) TTCACACGAGCTTCATCTGG | |||
| MDH (malate dehydrogenase) | (+) CCAACTGCAAGGTTCTGGTT | 124 | FE677903 |
| (−) GCCCTGTTGTGATCCAATCT | |||
| STOP1 (transcription factor) | (+) GCTCTAACTGCCGATGAGAA | 159 | SSH library |
| (−) TCTCTCCAGCTCCTCCTGAA | |||
| MATE-a (citrate transporter family) | (+) GCTGGATGCAGTTTCAAGAGAG | 138 | CV535133 |
| (−) ACTCCAGCAGCTGCAAGTTC | |||
| MATE-b (citrate transporter family) | (+) TGCTGTTCAAGCCATTCTAGC | 124 | CV534527 |
| (−) TCCAACAGCAAGAGAGAGTCC | |||
| MATE-c (citrate transporter family) | (+) GTGACACTGGCTGCATCATT | 91 | FD792891 |
| (−) GAGAAACTGCCAACCAAACC | |||
| ALS3 (Al-sensitive3) | (+) ACAAGCTTGGCCTCCAGATA | 106 | CV532021 |
| (−) GCGTTGTCCTGGTTGAAGAT | |||
| ALMT1 (Al-activated malate transporter) | (+) TTCGCCCCATCTGGGCTGGT | 118 | CV543751 |
| (−) TCCGGGGTTTCACTGCCATCA | |||
| VDAC (voltage dependent anion channel) | (+) TGCCTCGTTGACTCTGAATG | 146 | SSH library |
| (−) CCGAGGTACCAAGGATGTGA | |||
| ACC-oxidase† | (+) GAAGATGGCGCAAGAAGAAG | 105 | SSH library |
| (−) TGGAGCAAAGGTTCAAGGAG | |||
| β-Tubulin | (+) CCGTTGTGGAGCCTTACAAT | 117 | CV530631 |
| (−) GCTTGAGGGTCCTGAAACAA |
* (+) and (−) indicate forward and reverse primers, respectively.
† ACC-oxidase = aminocyclopropane-1-carboxylic acid oxidase.