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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Immunol. 2010 May 12;184(12):6766–6772. doi: 10.4049/jimmunol.0904054

FIGURE 3.

FIGURE 3

Apoptosis and cell loss in Trail−/− mice. A, Trail+/− or Trail+/+ (C57BL/6 littermates) were subjected to CLP or sham surgery. Twenty-four hours later, spleens were harvested, and TUNEL stains were performed and analyzed by flow cytometry on gated CD3+ cells. Results are reported as % TUNEL+ CD3+ splenocytes. B–D, Trail−/− or Trail+/− littermates were subjected to CLP or sham surgery. The number of CD4+ and CD8+ CD3+ T cells (B), splenic macrophages (CD11b+, F4/80+) (C), and DCs (CD11c+, MHC II+) (D) were quantified by flow cytometry. *Significant reduction versus Trail+/− mice.