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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Metab. 2009 Nov;10(5):379–391. doi: 10.1016/j.cmet.2009.10.003

Figure 6. The AQP-1/glycerol channel acts as a feedback regulator in the insulin/IGF-1 signaling pathway.

Figure 6

(A) aqp-1 mutation significantly decreased the mRNA level of a direct DAF-16 target gene, sod-3. (B) Expression of the insulin-like peptide gene ins-7 was induced by aqp-1 mutation. Error bars represent s.e.m. *P<0.05, two-tailed Student's t-test. These changes in sod-3 and ins-7 expression by aqp-1 mutation were not additive to daf-16(-) or hsf-1(-) mutations (Figure S16). (C) aqp-1 mutant animals that express sod-3::GFP generally contain GFP in the pharynx but not in the surrounding head muscles. (D, E) Some animals that express sod-3::GFP in a wild-type or in a Pges-1::GFP::daf-16 transgenic animal background contain GFP throughout the head, and often contain higher levels of GFP in the pharynx and intestine as well. The upper edge of the animal's head is indicated by the dashed yellow line. Arrowheads indicate head muscles. (F, G) Quantification of head-muscle sod-3::GFP expression for panel D-E without (F) or with (G) Pges-1::GFP::daf-16 [Pges-1::daf-16] (n≥75, *P<0.05, ***P<0.001, χ2 test (Tullet et al., 2008).