Skip to main content
. 2010 Jul;24(7):2281–2291. doi: 10.1096/fj.09-143487

Figure 3.

Figure 3.

Binding and endocytosis of 125I-leptin in HEK293 cells overexpressing mutant ObR constructs. A) Cell surface binding of 125I-leptin at 4°C was increased in all groups transfected with truncated ObRs, as well as ObRa or ObRb. Higher binding was seen in ObR865 and ObR875 than in all other groups. ObRa and ObR860 had the second highest percentage of leptin binding, whereas ObR1137 and ObRb had the least increase in comparison with the pcDNA vector control. B) Percentage of leptin binding correlated linearly with the level of ObR expression in groups of cells (r=0.91, P<0.001). C) Cell surface binding and endocytosis of 125I-leptin after incubation at 37°C for 30 min. D) Percentage of leptin endocytosis correlated linearly with that of residual cell surface binding (r=0.98, P<0.0001).