Figure 4.

Comparison of ObR860 and ObRa in mediating leptin trafficking in HEK293 cells. A) Most ObR860 immunoreactivity (green) was located at cell surface after leptin stimulation for 2 min, at which time Alexa555-leptin (red) showed complete colocalization with the receptor. At 15 min, Alexa555-leptin remained colocalized with ObR860, but mainly intracellularly. B) Immunoreactivity of ObRa was present both at cell surface and in cytosplasmic vesicles 2 min after leptin treatment. At this time, Alexa555-leptin (red) showed complete colocalization with ObRa (green). Fifteen minutes later, most of the Alexa555-leptin was colocalized with ObRa in intracellular vesicles.