Figure 1.

Increased levels of mir-146a in HIV-1-infected microglial cells. A) ELISA to detect p24 in medium collected at indicated time points from 2 different preparations of HIV-1-infected primary human fetal microglia (brains 7739 and 7490). B) qRT-PCR to detect levels of mature mir-146a in HIV-1-infected and control microglial cells at 0, 4, 8, and 12 d postinfection. qRT-PCR was performed on 25 ng of template, each in quadruplicate. Each sample was normalized for corresponding values of U6 control. Relative amount of mir-146a was calculated by the Roche LightCycler software. Graph represents average values obtained from microglial cell cultures isolated from brain samples 7739 and 7490.