Figure 7.
mir-146a is up-regulated in HIVE brain samples. A) qRT-PCR of mir-146a on RNA samples obtained from 4 HIVE patients (HIVE 030022, 010063, and 01057), 4 control brains (CB), and 2 HIV patients without brain pathology (HIV). qRT-PCR of mir-146a was performed in quadruplicate using 25 ng of RNA. B) Detection of hsa-mir-146a in HIVE 010063 and control CB 528B archived frozen samples. In situ hybridization was performed with miRCURY-LNA-DIG mir-146a (and control mir-128a) detection probes. Arrows indicate positive signal in cytoplasm of neurons. Original view: ×1000.