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. 2010 Jul;24(7):2334–2346. doi: 10.1096/fj.09-147991

Figure 4.

Figure 4.

Decreased repair activity in CSB-deficient mitochondrial extract. Incision of whole mitochondrial extract (WM) from CS1AN cells stably transfected with either empty vector (CSB-deficient) or CSB wild-type expression vector (wt). A) Incision represented as percentage cleaved substrate containing 8-oxoG, uracil, or 5-OH-U in a double-stranded oligodeoxynucleotide; 8-oxoG or 5-OH-U in an 11-nt bubble; or THF, an AP site analog, in a double-stranded oligodeoxynucleotide (Table 1). Results are means ± sd for 3–4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. wt. B) Representative gel picture for THF incision assay using WM from CSB wild-type cells and CSB-deficient cells, with increasing amounts of mitochondrial extracts from left to right.