Figure 5.

Prevention of CD4⁻8⁻ T-cell regulation of mixed CD4 + CD8 T-cell generation of IFN-γ by antagonism of cytokines. Columns and bars depict mean ± sd change induced by adding anti-cytokine/cytokine receptor antibodies and/or the MIF receptor antagonist Iso-1 (I) to CD4 + CD8 T cells mixed with 3% CD4⁻8⁻ T cells from 3 healthy donors before stimulation by anti-CD3 + anti-CD28 antibodies (no additions control=100%). Antibodies were to Serpin E1 (S; 2 μg/ml), RANTES (R; 10 μg/ml), and MIF + MIF receptor (M; 30 and 6 μg/ml, respectively); Iso-1 (I) level was 40 μM. Bars in each pair depict data from d 2 and 3 in that order. Statistical analyses and symbols as in Fig. 2, except that brackets over Iso-1 data in left frame show significantly greater inhibition at both days when S is added to Iso-1.