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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biochemistry. 2010 Jun 1;49(21):4466–4475. doi: 10.1021/bi902213r

Figure 3.

Figure 3

Positive ESI LC/MS mass chromatograms of hydroxyraloxifene metabolites (490 m/z) from incubations of raloxifene with CYP3A4. The top chromatogram shows that both 7-OHRA and 3′-OHRA are detected after the incubation was terminated with TCA. The middle chromatogram shows that only 3′-OHRA was detected after the incubation was terminated with methanol. In the bottom chromatogram, the protein pellet from the methanol termination was resuspended in a TCA solution, and both 3′-OHRA and 7-OHRA were recovered from the protein pellet.