Skip to main content
. Author manuscript; available in PMC: 2011 Jun 11.
Published in final edited form as: Cell. 2010 Jun 11;141(6):1030–1041. doi: 10.1016/j.cell.2010.04.036

Figure 4. POM121 precedes the Nup107/160 complex during interphase NPC assembly.

Figure 4

(A) Assembled nuclei were incubated with mock- or WGA-depleted cytosol for 30 min in the presence of buffer, BAPTA, IgG or POM121 Fab fragments. Fluorescently labeled 70 kDa dextran (green) was added to probe for INM-ONM fusion. (B) Quantification of intranuclear 70 kDa dextran signal in conditions described in (B), N>150 nuclei per condition. (C) Immunofluorescence staining of the nuclear surface using specific antibodies against POM121 and Nup107. Circle indicates NPC intermediate containing POM121 but not Nup107, scale bar 1 μm. (D) Quantification of NPC intermediates in control (scrambled) and Nup107 or Nup96 RNAi-treated cells, n = 30 fields. See also Figure S4.