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. 2010 Jun 17;5(6):e11167. doi: 10.1371/journal.pone.0011167

Figure 1. Morphological changes of hES cells after treatment with BMPs.

Figure 1

H9 cells were treated with BMP-2, BMP-6 or BMP-2/6 in mTeSR1 for 5 days. Noggin 1 µg/ml was used as antagonist to BMP-2. Medium was changed daily for fresh medium supplemented with the desired concentration of agonists. Pictures were taken using an inverted microscope and 10X objective. A, control cells in mTeSR1. B, BMP-2 100 ng/ml. C, BMP-6 100 ng/ml. D, BMP-2/6 100 ng/ml. E, BMP-2 100 ng/ml + noggin 1 µg/ml. F, BMP-2 10 ng/ml. G, BMP-6 10 ng/ml. H, BMP-2/6 10 ng/ml. Arrowheads indicate morphologically pluripotent cells; arrows point out morphologically differentiated areas. Insets belong to 3× magnification.