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. 2010 Jun 17;5(6):e11167. doi: 10.1371/journal.pone.0011167

Figure 5. Expression analysis of endodermal marker CXCR4 in hES cells treated with BMPs.

Figure 5

H9 cells were treated with BMP-2, BMP-6 or BMP-2/6 in mTeSR1 for 1, 3 or 5 days. Noggin 1 µg/ml was used as antagonist. After 5 days of treatment, qPCR and flow cytometry were used to analyze expression of the endodermal marker CXCR4. qPCR values correspond to relative expression compared to GAPDH mRNA. As control, cells growing in mTeSR1 were used. Treatments were repeated at least in three different experiments, and results are expressed as average ± SD. A, qPCR analysis of CXCR4 expression. B, Flow cytometry analysis of CXCR4 after incubation with BMP-2, BMP-6 or BMP-2/6 at 10 or 100 ng/ml. C, D, Flow cytometry histograms of CXCR4-positive cells after incubation with BMPs at 10 (C) or 100 ng/ml (D). As negative controls, untreated cells (TeSR) and PE-conjugated mouse IgG2a isotype control (isotype) were used. (*) P<0.05 BMP-2 vs. BMP-2/6.