Table 1.
In Vitro Activity of Compounds 4–10 in Cytotoxicity (HT-29 Cell Line) and ELISA NF-κB (p65 and p50) Assays.
| compound | 4 | 5 | 6 | 7 | 8 | 9 | 10 | camptothecind | rocaglamided |
|---|---|---|---|---|---|---|---|---|---|
| cytotoxicitya | 4.9 | 1.7 | 1.7 | 2.3 | 9.1 | >10 | >10 | 0.06 | - |
| NF-κB (p65)b | NTc | 15.9 | 12.1 | 3.2 | >20 | 11.3 | 19.0 | - | 0.08 |
| NF-κB (p50)b | NTc | >20 | 7.5 | >20 | >20 | >20 | >20 | - | 2.0 |
a
Compounds 1–3 and 11 were inactive (ED50 >10 μM).
b
Compounds 1, 2, and 11 were inactive (IC50 >20 μM).
c
Compounds 3 and 4 were not tested in the NF-κB assays because of the limited amounts available.
d
Positive control substances.