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Relative quantification of GPNMB expression in stomach cancer cell lines depends on different reference genes. The GPNMB expression in six stomach cancer cell lines were normalized by six single reference genes and best combination derived by geNorm (mean ± SD); normalized by B2M (A), by GAPDH (B), by RPL29 (C), by 18S rRNA (D), by ACTB (E), HPRT1 (F) and geometric mean of B2M-GAPDH combination (G).
