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Relative quantification of GPNMB expression in normal and tumor stomach tissues depends on different reference genes. The GPNMB expression in stomach cancer tissues (solid bar: Normal, open bar: Tumor) were normalized by six single reference genes and two combinations derived from geNorm and NormFinder (mean ± SD); normalized by RPL29 (A), by HPRT1 (B), by B2M (C), by 18S rRNA (D), by ACTB (E), GAPDH (F), geometric mean of RPL29-HPRT1 (G) and RPL29-B2M (H).
