Table 1.

Pairwise comparisons used for the microarray experiments

Genotype	Sex	Cy3 Germline status	Cy5 Germline status	Notes
tud1 bw1 sp1/tud1 bw1 sp1	male	conditioned	naive	a
tud1 bw1 sp1/tud1 bw1 sp1	male	naïve	conditioned	a
tud1 bw1 sp1/CyO-DTS	male	conditioned	naïve	b
tud1 bw1 sp1/CyO-DTS	male	naïve	conditioned	b
gs(1)N441/Y	male	conditioned	naïve	c
gs(1)N441/Y	male	naïve	conditioned	c
tud1 bw1 sp1/tud1 bw1 sp1	female	conditioned	naïve	a
tud1 bw1 sp1/tud1 bw1 sp1	female	naïve	conditioned	a
tud1 bw1 sp1/CyO-DTS	female	naïve	conditioned	b
tud1 bw1 sp1/CyO-DTS	female	conditioned	naïve	b
tud1 bw1 sp1/CyO-DTS	female	conditioned	naïve	a
tud1 bw1 sp1/CyO-DTS	female	naïve	conditioned	a
gs(1)N441/gs(1)N441	female	conditioned	naïve	c
gs(1)N441/gs(1)N441	female	naïve	conditioned	c

^a Separate cultures for germline-conditioned and naïve flies, allowed to mate.

^b Grown in same culture, isolated sexes as virgins and cultured separately for 3-5 days.

^c Grown in same culture, allowed to mate.