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. 2010 May 9;7:89. doi: 10.1186/1743-422X-7-89

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Copyright ©2010 Zhang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Titers of antibody against sM2 in SPF chicken serum detected by ELISA. Seventy-five SPF chickens aged 3 weeks were divided into 5 (n = 15) groups. The chickens in the five test groups were immunized thrice with GST-sM2, GST-C3d-L1-C3d-L2-sM2, pcDNA-sM2, pcDNA-C3d-L1-C3d-L2-sM2 and GST-sM2+IFA, respectively. Blood samples were collected before each vaccination and two weeks after the final booster, and antibody against sM2 were detected by ELISA. Ab-positive cut-off values were set as mean + 2 SD of non-immunized sera. An ELISA Ab titer was expressed as the highest serum dilution giving a positive reaction. The titer values were showed in 100 (Log2X ± S).