Table 1.
Proteins of the glycolysis pathway identified in H. influenzae strain 11P6H identified during growth in pooled human sputum
| Protein ID# | Identified Protein | Gene | Genome ID numbera | Molecular Weight | CDMb | Sputumc |
|---|---|---|---|---|---|---|
| 1237 | phosphoenolpyruvate-protein phosphotransferase | fruA | HI0446 | 64 kDa | 100% (9.7%) | 100% (11%) |
| 412 | Fructose specific phosphotransferase system IIA/HPr components | fruB | HI0448 | 53 kDa | 100% (24%) | 100% (8.2%) |
| 1149 | Aldose 1-epimerase | galM | HI0818 | 38 kDa | 100% (11%) | 100% (15%) |
| 423 | 1-phosphofructokinase | fruK | HI0447 | 34 kDa | 100% (24%) | 100% (15%) |
| 557 | 6-phosphofructokinase | pfkA | HI0982 | 23 kDa | 100% (21%) | 95% (20%) |
| 57 | Fructose-bisphosphate aldolase | fba | HI0524 | 39 kDa | 100% (47%) | 100% (36%) |
| 657 | glucose-6-phosphate isomerase | pgi | HI1576 | 37 kDa | 100% (19%) | 100% (15%) |
| 392 | Triosephosphate isomerase | tplA | HI0678 | 27 kDa | 100% (25%) | 100% (19%) |
| 97 | Glyceraldehyde-3-phosphate dehydrogenase | gapA | HI0001 | 36 kDa | 100% (40%) | 100% (39%) |
| 111 | 3-phosphoglycerate kinase | pgk | HI0525 | 41 kDa | 100% (39%) | 100% (37%) |
| 34 | phosphoglyceromutase | gpmA | HI0757 | 26 kDa | 100% (52%) | 100% (56%) |
| 79 | enolase | eno | HI0932 | 46 kDa | 100% (43%) | 100% (32%) |
| 133 | Pyruvate kinase | pykA | HI1573 | 49 kDa | 100% (37%) | 100% (47%) |
| 538 | Dihydrolipoamide acetyltransferase | aceF | HI1232 | 57 kDa | 100% (22%) | 100% (23%) |
| 305 | Pyruvate dehydrogenase subunit E1 | aceE | HI1233 | 99 kDa | 100% (28%) | 100% (30%) |
aID numbers based on annotation of H. influenzae strain KW20 Rd http://cmr.jcvi.org/cgi-bin/CMR/GenomePage.cgi?org=ghi
bProtein probabilities values as calculated by Proteinprophet algorithm for proteins detected during growth in chemically define media (CDM). Number in parentheses represents the sequence coverage expressed by the percentage of amino acid residues identified. All peptides were filtered with a set of criteria as specified in the Methods. CDM
cProtein probabilities for proteins detected during growth in 20% pooled human sputum.