Table 2.

Composition of chemically defined media (CDM)

Reagent	Concentration
NaCl	0.1 M
K2SO4	5.75 mM
Na2EDTA	4 mM
NH4Cl	4 mM
K2HPO4	2 mM
KH2PO4	2 mM
Thiamine HCl	6 μM
Thiamine pyrophosphate	1 μM
Pantothenic acid	8 μM
d-Biotin	12 μM
Glucose	0.5%
Hypoxanthine	0.375 mM
Uracil	0.45 mM
L-aspartic acid	3.75 mM
L-glutamic acid HCl	7.5 mM
L-arginine	0.875 mM
Glycine HCl	0.225 mM
L-serine	0.475 mM
L-leucine	0.7 mM
L-isoleucine	0.225 mM
L-valine	0.525 mM
L-tyrosine	0.4 mM
L-cysteine HCl	0.35 mM
L-cystine	0.15 mM
L-proline	0.45 mM
L-tryptophan	0.4 mM
L-threonine	0.425 mM
L-phenylalanine	0.15 mM
L-asparagine	0.2 mM
L-glutamine	0.35 mM
L-histidine HCl	0.125 mM
L-methionine	0.1 mM
L-alanine	1.125 mM
L-lysine	0.35 mM
Glutathione reduced	0.15 mM
HEPES	42 mM
NaHCO3	0.125 mM
Na acetate trihydrate	6.25 mM
Choline chloride salt	0.05 mM
Myo-inositol	1 μM
MgCl2	2.5 mM
CaCl2	0.6 mM
Fe(NO3)3	0.1 mM
Nicotinamide adenine dinucleotide	0.02 mM
Protoporphyrin IX	0.02 mM
Histidine	6 μM
Triethanolamine	0.01%