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. 2010 Jun 17;6(6):e1000968. doi: 10.1371/journal.ppat.1000968

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Erythrocytes from 27 healthy donors were used. To control for day-to-day variation the percent invasion as well as the CR1 MFI were normalized to an erythrocyte standard sample that was used in every experiment. The CR1 MFI was re-measured once in 4 samples with extreme values. In 19 donors the invasion assay was repeated 1–3 times. When more than one measurement was done, the mean was used for normalization.