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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Nat Immunol. 2010 Mar 28;11(5):395–402. doi: 10.1038/ni.1864

Figure 1.

Figure 1

Characterization of Aim2-genetrap mice. a Schematic of wild-type and gene-trapped Aim2 allele. b. RT-PCR analysis shown the absence of wild-type (WT) Aim2 transcripts in the Aim2-genetrap mice. WT Aim2 RNA was detected by primers F and R1 and gene-trapped Aim2 RNA was detected by primers F and R2. c. Immunoblotting of Aim2 protein in peritoneal and bone marrow derived macrophages from Aim2+/+ and Aim2-/- mice. Equal amounts of protein were loaded for all the samples and Aim2 was detected using a rabbit polyclonal antibody.