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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Nat Immunol. 2010 Mar 28;11(5):395–402. doi: 10.1038/ni.1864

Figure 2.

Figure 2

Aim2 is essential for inflammasome activation by DNA. a. 293T cells were transfected with indicated plasmids together with a NF-κB reporter and TK Renilla-luciferase reporter gene. After 48 h, luciferase activity was measured. b. 293T cells were transfected with the indicated plasmids together with murine pro-IL-1β-Gluc/Flag. After 24 h, cell lysates were immunoblotted for pro-IL1β-Flag, IL-1β-Flag and β-actin. c. F4/80 and CD11b expression by BMDM from Aim2+/+ and Aim2-/- mice. d-f. LPS (200 ng/ml) primed Aim2+/+ and Aim2-/- BMDM, thioglycollate-elicited macrophages (TEM), and BMDCs were transfected with poly(dA-dT) (0.75 – 3 μg/106 cells) for 6 h. The secreted IL-1β (d-e) and the cleaved forms of IL-1β and caspase 1 (f) in the culture supernatants were analyzed. g. Aim2+/+ and Aim2-/- BMDM were treated as above and the IL-18 levels in the supernatants at 6 h were measured by ELISA. (h). Aim2+/+ and Aim2-/- TEM were treated as indicated for 24 h and cell viability was reported as % of viability of medium treated cells. Asterisks indicate P values of < 0.05 for Aim2+/+ versus Aim2-/-. Data are presented as mean ± SD from one experiment representative of 3 experiments.