Changes in body weight and cell and protein levels in BALF after SDAV inoculation. The rats were infected in two cohorts. Cohort 1 was harvested on days 2, 4, 8 and 12 after instillation (n=4–5 rats per time point) and Cohort 2 was harvested at 0.5, 1 and 2 days after instillation (n=3–4 rats per time point). (a) Rat weights were recorded each day and lungs were harvested on days 0.5, 1, 2, 4, 8 and 12. (b, c and d) Cells from BALF were counted on a haemocytometer and scored on cytospin slides. (e) BALF protein levels were determined by bicinchonic acid protein assay. Asterisks indicate statistical significance (P<0.05) between SDAV and control samples.