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. 2010 Jan;59(Pt 1):8–16. doi: 10.1099/jmm.0.012864-0

Fig. 1.

Fig. 1.

Schematic representation of the approach used. The pYJTags was created from pUC19 with the insertion of a temperature-sensitive (ts) Myc ori. It allowed for the integration of the transposon once the environmental temperature was elevated to 41 °C.