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Expression of truncTAg from the cDNA clone. BSC cells were transfected with pcDNA3.1(+)-TAg, pcDNA3.1(+)-truncTAg or pcDNA3.1(+)-FLAGtruncTAg, and whole-cell lysates were prepared at 2 days p.t. Aliquots of total cell protein (30 μg) were separated on a 10 % SDS-PAGE gel and analysed by Western blot, probing with PAb416. *, A non-specific band of approximately 70 kDa; No Tfxn, untransfected cells; RPTE-BKV, BKV-infected RPTE whole-cell lysates.
