Figure 4.

Cycloheximide abrogates R1881 induction of CXCR4 gene expression. VCaP cells were treated with vehicle, R1881 (0.5 nM), cycloheximide (5 µg/ml), and a combination of cycloheximide and R1881. In the combination experiment, cycloheximide was initially treated with cells, and 1 hour later, R1881 was added. CXCR4 and PSA gene expressions were determined by quantitative PCR. Fold differences in the gene expression were determined after normalization with GAPDH expression.