Table 2.
Genetic interactions between let-7 and the translation machinery
| Translation factor | locus | ORF | let-7(n2853) suppression |
|---|---|---|---|
| Initiation factors | |||
| eIF1 | T27F7.3b | − | |
| eIF1Aa | H06H21.3 | ++ | |
| eIF2βb | iftb-1 | K04G2.1 | ++ |
| eIF2γd | Y39G10AR.8 | × | |
| eIF2A | E04D5.1 (a, b) | − | |
| eIF2Bα | ZK1098.4 | − | |
| eIF2Bβ | Y47H9C.7 | ++ | |
| eIF2Bγ | ppp-1 | C15F1.4 | ++ |
| eIF2B∂ | F11A3.2 | +++ | |
| eIF2Bεd | D2085.3 | +++ | |
| eIF3ac,d | egl-45 | C27D11.1 | × |
| eIF3bc,d | eif-3.B | Y54E2A.11a | +++ |
| eIF3cc,d | eif-3.C | T23D8.4 | +++ |
| eIF3dd | eif-3.D | R08D7.3 | ++ |
| eIF3ed | eif-3.E | B0511.10 | +++ |
| eIF3fd | eif-3.F | D2013.7 | +++ |
| eIF3gd | eif-3.G | F22B5.2 | × |
| eIF3h | eif-3.H | C41D11.2 | ++ |
| eIF3ib | eif-3.I | Y74C10AR.1 | × |
| eIF3k | eif-3.K | T16G1.11 | − |
| eIF3ma | cif-1 | K08F11.3 | +++ |
| eIF4Ab,d | inf-1 | F57B9.6 | × |
| eIF4Aa,d | F57B9.3 | +++ | |
| eIF4E-1+5 | ife-1 | F53A2.6 | − |
| ife-5 | Y57A10A.30 (a, b) | ||
| eIF4E-2 | ife-2 | R04A9.4 | − |
| eIF4E-3 | ife-3 | B0348.6 (a, b, c) | − |
| eIF4E-4 | ife-4 | C05D9.5 | − |
| eIF4Gb,d | ifg-1 | M110.4 (a, b) | × |
| eIF4H | drr-2 | T12D8.2 | − |
| eIF5b | C37C3.2 (a, b, c) | ++ | |
| eIF5Ad | iff-2 | F54C9.1 | +++ |
| eIF5B | iffb-1 | Y54F10BM.2 | +++ |
| eIF6 | eif-6 | C47B2.5 | ++ |
| Elongation factors | |||
| eEF1Ab,d,e | eft-3 | F31E3.5 | ++ |
| eft-4 | R03G5.1 (a, b, c, d) | ||
| eEF1Be | Y41E3.10 | ++ | |
| F54H12.6 | |||
| eEF2b,d | eft-2 | F25H5.4 | +++ |
| eEF2c,d | eft-1 | ZK328.2 | ++ |
| Release factors | |||
| eRF1c,d | T05H4.6 (a, b) | × | |
| eRF3b,d | H19N07.1 | ++ |
In some cases, RNAi titration was performed to overcome developmental block by mixing bacteria that carried the dsRNA producing plasmid with those carrying a plasmid without insert.
a
1:1 dilution
b
1:5 dilution
c
1:10 dilution
d
slow growth
e
multiple RNAi targets. Suppression: −,<20%, +, >20%, ++, >40%, +++, >80%, x, developmental block, n ≥ 60 worms for each. Control animals fed with bacteria carrying empty L4440 vector showed never more than 10% survival, whereas daf-12(RNAi), our positive control, showed never less than 90% survival.