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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Mar;67(3):193–206. doi: 10.1002/cm.20437

Figure 2.

Figure 2

WASH is widely expressed in mammalian tissues and cell lines and fractionates with both cytosol and membranes. (A) Cell extracts from mouse tissues or (B) from mammalian cultured cell lines were separated by SDS-PAGE and immunoblotted with anti-WASH antibodies. (C) NIH-3T3 cells were fractionated into membrane and cytosolic fractions by centrifugation and then separated by SDS-PAGE and immunoblotted with anti-WASH antibodies, antibodies against tubulin (cytosolic fraction marker), or antibodies recognizing calnexin (membrane fraction marker).