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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Mar;67(3):193–206. doi: 10.1002/cm.20437

Figure 5.

Figure 5

Disruption of the actin cytoskeleton results in an enlarged and elongated WASH-containing and EEA1-positive endosomes. (A) WASH or (B) EEA1 visualized by immunofluorescence (green), F-actin stained with Alexa Fluor 568-phalloidin (red) and DNA stained with DAPI in COS7 cells treated with DMSO alone, or DMSO with 10 μM cytochalasin D (Cyto D), or 10 μM latrunculin A (Lat A). Imaging was by 3D structured illumination microscopy. Each panel is a representative maximum intensity projection of the 3D images. Actin colocalized with WASH structures (see arrows) in control (DMSO) treatment. Scale bars, 5 μm.