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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Mar;67(3):193–206. doi: 10.1002/cm.20437

Figure 6.

Figure 6

WASH and the Arp2/3 complex control endosome shape. (A) Extracts from fibroblast-like cells (FLCs) treated with control non-specific siRNAs, siRNAs targeting the Arp3 and ARPC3 subunits of the Arp2/3 complex, or WASH siRNAs were separated by SDS-PAGE and immunoblotted with anti-Arp3, anti-WASH, or anti-GAPDH (loading control) antibodies. (B) RNAi silencing of Arp2/3 complex expression in FLCs lead to enlarged WASH-positive compartments visualized by immunofluorescence. (C) RNAi silencing of WASH in FLCs lead to enlarged EEA1-positive endosomes visualized by immunofluorescence. Scale bars, 10 μm.