Skip to main content
. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Mar;67(3):193–206. doi: 10.1002/cm.20437

Figure 7.

Figure 7

WASH depletion does not affect the kinetics of transferrin uptake or recycling in the presence or absence of N-WASP. (A) Extracts from NIH-3T3s or fibroblast-like cells (FLCs) transfected with control or WASH siRNAs were separated by SDS-PAGE and immunoblotted with anti-WASH or anti-GAPDH antibodies. (B) Single-round kinetics of uptake and recycling of prebound biotinylated transferrin (B-SS-Tfn) in NIH-3T3 cells, N-WASP+/+ FLCs, and N-WASP-/- FLCs. Graph shows percent of total B-SS-Tfn that is internalized (mean of 3 independent experiments ± SD) versus time. (C) WASH (green) and N-WASP (red) stained by immunofluorescence and DNA (blue) stained by DAPI were visualized in COS7 cells by deconvolution microscopy. Scale bar, 5 μm.