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. Author manuscript; available in PMC: 2011 Jun 11.
Published in final edited form as: Circ Res. 2010 Apr 22;106(11):1763–1774. doi: 10.1161/CIRCRESAHA.109.213025

Figure 1. Nox4 is the most abundant Nox homologue in the PVN and is selectively upregulated following MI.

Figure 1

A) Summary of real-time RT-PCR analysis showing basal copy numbers of Nox1, Nox2, and Nox4 in PVN micropunches of naïve mice (n=6, each sample pooled from 3-4 mice); *p<0.05 vs. Nox1 or Nox2. B) Summary of Nox4 mRNA levels in PVN 14 days after MI expressed relative to sham mice (n=6 per group, each sample pooled from 3-4 mice); *p<0.05 vs. sham. C) Representative DIG staining (from 3 experiments) in PVN following in situ hybridization with antisense cRNA probes targeted against Nox4 from mice 14 days post-MI or sham surgery. MI mice had undergone PVN injections of AdsiGFP or AdsiNox4 three days before MI or sham. D) Representative Western blot (upper panel) and summary data (lower panel) of Nox4 protein levels from mice with or without PVN-targeted gene transfer of AdsiGFP or AdsiNox4 and 14 days after MI or sham surgery (n=4 per group, each sample pooled from 3 mice). *p<0.05 vs. Sham; †p<0.05 vs. MI alone and MI+AdsiGFP.