Figure 6. Role of G_i/o-signaling but not PAF receptors.

Data are from intracellular Ca²⁺ measurements from TRPC5-expressing HEK 293 cells (a-c) and VSMCs (d-f). Cells were pretreated with 1 μg ml⁻¹ pertussis toxin (PTX) or denatured (denat.) PTX for 4 hr at 37 °C. (a-c) Responses were evoked by 3 μM PGPC (a, n/N=3/9), 5 μM POVPC (b, n/N=3/9) or 5 μM lysophosphatidylcholine (c, n/N=3/18). (d) Representative paired experiment showing responses to 3 μM PGPC after treatment with PTX or denatured PTX (N=5 each). (e) Responses were evoked by 3 μM PGPC or 5 μM POVPC (n/N=4/20 each). (f) Responses to 3 μM PGPC in cells pre-incubated and maintained in the presence of 100 μM WEB2086 or its vehicle (N=6 each and representative of 3 independent experiments).