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Purification of His-tagged IFS protein. The protein overexpressed with IPTG in E. coli JM109 having pHis-IFS (lane 2), but not in E. coli JM109 having the control vector pQE80L (lanes 5 and 6), was purified as shown in lane 3. The protein was detected by anti-RGS:His antibody to confirm the expected His-tagged product (lane 7).
