Table 3.
Virulence (Mortality) to mouse of GT01Δnga with or without cloned nga gene
| Strain | Mortalitya | NADaseb |
|---|---|---|
| GT01 (pLZ12-Km2, vector) | 73% (8/11) | 14.12 ± 1.30 |
| GT01Δnga (pLZ12-Km2, vector) | 0% (0/17) | 0.04 ± 0.06 |
| GT01Δnga (pLZN2, nga) | 0% (0/11) | 1.28 ± 0.12 |
| GT01Δnga (pLZN-RBS, nga) | 0% (0/10) | 1.78 ± 0.03 |
| GT01Δnga (pLZN-RBSII2, nga) | 29% (4/14) | 4.57 ± 0.17 |
Bacteria were cultured in BHI-Y broth supplemented with kanamycin (100 μg/ml).
a, Mice were observed for 8 days, because no mouse died after day 8 on previous study (see Figure 1).
b, NADase activity was determined as described in Table 2.