Figure 1. The effect of lethal intranasal infection with 100×LD₅₀ LVS on activation and recruitment kinetics of lymphocytes in the respiratory system.

(A) Survival of 60 C57BL/6 mice in after intranasal infection with 100×LD₅₀ LVS (Accumulation of six independent experiments with 10 mice in each experiment); (B) Live bacterial counts in lungs (dark bars) and mediastinal lymph nodes (MdLN) (light bars) at the indicated time points after infection. The average count in three animals is shown in each time point. (C) CD69 expression analysis on gated NK cells (NK1.1⁺CD3⁻ cells) in the lungs (closed diamonds) and MdLN (open squares) at the indicated time points after infection (D) CD69 expression analysis on gated T cells (NK1.1⁻CD3⁺ cells). The respective insets in both (C) and (D) show the total numbers of NK or T cells; Cells were pooled from three animals at each time point and analyzed; (E) CD69 expression on gated NK and T cells in the lungs or MdLN three days following instillation of PBS (white bars), 100×LD₅₀ live LVS (black bars) or equivalent dose of inactive LVS (gray bars). Cells were analyzed from three pooled animals. Panels B-E depict a representative experiments out of three independent experiments performed. Each time point included three animals.