Table 1. Primers used for large-scale genotyping.

PCR #	Locus	Primers sequence	Primer amount (pmol)	Label	Repeat Motif	Range (bp)	Allele Count	Ho	He	Percent missing
1	euphy 2	tgatgataacgagcgggaag	0.5	5′TAM	CAG	144–191	20	0.42	0.72	0.60%
		cggtaccgctacgtgactact
	euphy 3	gctgtaatttggtaaggggttg	0.5	5′ HEX	ATC	121–171	18	0.52	0.83	0.84%
		tacgttcagtgatggacatgc
	euphy 21	acgcaaggtgctccacttat	0.5	5′ HEX	CAA	220–239	9	0.18	0.24	1.32%
		ttgctacgctaacagcatcg
	euphy 69	ctcctccgcaccaacaagta	1	5′ FAM	GTT	72–103	13	0.17	0.39	3.59%
		aaacgtctacgttagaaggtatgt
2	euphy 14	tgactgaacacacggacgat	0.5	5′ TAM	TACA	99–170	32	0.15	0.68	14.0%
		tccatcatgctttaagtgagga
	euphy 61	aaagcgtgcttacattacatgg	0.5	5′ TAM	AC	186–246	42	0.44	0.87	12.9%
		tcccgtttaacataatctgtgg
3	euphy 35	atagaaataaacatgcggccata	10	dUTP	TG	267–335	56	0.33	0.96	13.1%
		cagatgtacaagaggctgcctta
	euphy 50	atgcgatttcatgccacata	10	dUTP	CA, A	135–176	28	0.22	0.85	22.5%
		ccatcctgacatgtgaaacg
4	euphy 37	tgcaagacttgaaatatggttatca	10	dUTP	C, CA	130–182	21	0.41	0.80	2.28%
		gtccattggaaggatcagga
	euphy 47	cacgtgagcattccagtttg	10	dUTP	AT	172–335	34	0.44	0.87	5.99%
		tcggcgtaacggtttaaatg

Summary statistics are based on a survey of 835 individuals from 72 populations (Table 1). Even and odd numbered reactions were pooled and analyzed together in the same sequencer run. The percentages of missing were significantly different among the PCR mixes, being significantly higher in reactions 2 and 3 (F_3,6 = 15.4, p = 0.0038).