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. 2010 Jun 18;5(6):e11204. doi: 10.1371/journal.pone.0011204

Figure 6. Syntaxin 1B is the interacting Q-SNARE partner of SNAP 23 and VAMP 3 upon capacitation.

Figure 6

(A): In control sperm syntaxin 1B appeared predominantly in the cavitated membrane fraction (285000 g) but also in the remaining cavitated sperm head fraction (1000 g) in the free 36 kDa form. However, a 80 kDa SDS-resistant syntaxin 1B-containing protein complex emerged in the non-reducing cavitated membrane fraction of capacitated sperm cells indicating its capacitation-dependent interaction with other proteins. This 80 kDa protein complex shows the same 0.1 M DTT and thermal stability (up to 90°C) as the SNAP 23 and VAMP 3-containing protein complexes (Figs. 4). Experimental set ups were identical as in Figs. 4 and 5. (B): Western blot data of non-reducing samples revealed that the presence of a 80 kDa protein complex is exclusive for the cavitated membrane fraction from capacitated sperm (285000 g pellet). WC =  whole cell lysate; 1000 g =  remaining cavitated head fraction; 285000 g =  cavitated membrane fraction. 10 µg of total protein extract was used for all samples. Pig brain homogenate was used as positive control.