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. 2010 May 28;8:55. doi: 10.1186/1477-7827-8-55

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Copyright ©2010 O'Brien et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Representative Western blot of GHS-R1 protein expression of β-Estradiol 10^-8M (BE) treated hTERT-HM cells compared to control (C) untreated cells. The corresponding ACTB western blot is presented underneath the western blot. Molecular weights are indicated in kDa. Quantitative densitometric analysis of this western blot is presented, with β-Actin-normalised densitometric units for prepro-ghrelin plotted against treatment ± SEM (indicated with error bars). ** indicates a significance value of P < 0.01.