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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Biomaterials. 2010 Feb 2;31(12):3185–3200. doi: 10.1016/j.biomaterials.2010.01.041

Figure 1.

Figure 1

Immunophenotyping of undifferentiated rat BMSCs by flow cytometry. Single parameter histograms showing the relative fluorescence intensity of staining (abscissa) and the number of cells analyzed, events (ordinate). Isotype controls were included in each experiment to identify the level of background fluorescence. The intensity and distribution of cells stained for hematopoietic and endothelial markers; CD11b, CD31, CD34, CD44, CD45, CD106 and OX43 (black, shaded peaks) were not significantly different from those of isotype control (grey, shaded peaks) (Panels A-E, H-I), indicating that these cultures were devoid of any potential hematopoietic and/or endothelial cells of bone marrow origin. The fluorescent intensity was greater (shifted to right) when BMSCs were stained with CD73 and CD90 (black) compared to isotype control (grey) (Panels F, G). The predominant population of BMSCs consistently expressed CD90 surface molecule, a property of rat bone marrow-derived mesenchymal/stromal stem cells.