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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Biomaterials. 2010 Feb 2;31(12):3185–3200. doi: 10.1016/j.biomaterials.2010.01.041

Figure 3.

Figure 3

Expression pattern of various cardiomyogenic markers in ECMs containing myotube constructs by confocal microscopy. Localization of key cardiac myocyte phenotypic markers of day 21 embryonic cardiac myocyte (ECM) tube cultures in basal medium demonstrated the expression of structural and contractile proteins, sMHC (A, C), cTnI (D, F), α-actinin (G, I), microfilament, actin (B-C, E-F, H-I, K-L) and the adherent junction protein, N cadherin (J, L). Dual immunostaining of ECMs tube cultures in basal medium revealed areas of elongated and/or round to polyhedral type of cells arranged in multilayers and organized into cord-like or trabecular type of cellular arrangement (A-L). Nuclei of these cells were large and either oval or elongated and fusiform in appearance and were centrally positioned. Cells were also stained for nuclei (blue, DAPI) and fibrillar actin (green, Alexa 488 phalloidin). Merged images (A-L). (A-C, scale bar 100 μm; D-L, scale bar 50 μm).