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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Biomaterials. 2010 Feb 2;31(12):3185–3200. doi: 10.1016/j.biomaterials.2010.01.041

Figure 9.

Figure 9

Nuclear morphometric analysis of BMSCs, ECMs and ECMs/BMSCs cultured in the tubular scaffold under basal conditions. The descriptors of nuclear morphology, viz., area - A (A), perimeter - P (B), the equivalent oblate volume – EOV (C) and the elliptical form factor – EFF (D) were measured by the imaging software for each thresholded and image isolated nucleus. Values of A, P, EFF and EOV were calculated directly from the integrated morphometry subroutine of Meta-Morph image analysis software. The nuclear parameters (A, P, EOV and EFF) of BMSCs nuclei were significantly greater than the nuclear parameters of ECMs and ECMs/BMSCs nuclei (p < 0.001). There were no differences between the nuclear parameters (P, EOV and EFF) for the ECMs and ECMs/BMSCs nuclei (p = 0.101, p = 0.426, p = 0.657), except the nuclear area (ECMs > ECMs/BMSCs, p = 0.008). The data represent the means ± SEM. *p < 0.05; **p < 0.001.