Table 1. Primary antibodies used in this study.
Primary Antibodies | Dilutions | Source | Cell Target |
---|---|---|---|
BMSCs characterization markers | |||
CD11b | 1:50 | BD Pharmingen | Leukocytes |
CD31 | 1:10 | Abcam | Endothelial |
CD34 | 1:50 | Santa Cruz Biotechnology | Endothelial |
CD44 | 1:10 | Gene Tex, Inc | Leukocytes |
CD45 | 1:50 | BD Pharmingen | Hematopoietic |
CD73 | 1:50 | BD Pharmingen | BMSCs |
CD90 | 1:50 | BD Pharmingen | BMSCs |
CD106 | 1:50 | BD Pharmingen | Endothelial |
OX43 | 1:10 | Gene Tex, Inc | Endothelial |
Cardiac myocyte differentiation markers | |||
Sarcomere myosin (MF20) | 1:100 | DSHB | Cardiomyocyte |
Cardiac myosin heavy chain (α/β MHC) | 1:100 | Abcam | Cardiomyocyte |
Cardiac troponin I (cTnI) | 1:100 | Santa Cruz Biotechnology | Cardiomyocyte |
Cardiac α-actinin | 1:100 | Santa Cruz Biotechnology | Cardiomyocyte |
Connexin 43 (Cx43) | 1:100 | Santa Cruz Biotechnology | Cardiomyocyte |
N Cadherin | 1:100 | Santa Cruz Biotechnology | Cardiomyocyte |
Desmin | 1:100 | Abcam | Cardiomyocyte |
GATA4 | 1:100 | Santa Cruz Biotechnology | Cardiomyocyte |
ANP | 1:100 | Santa Cruz Biotechnology | Cardiomyocyte |
BNP | 1:100 | Santa Cruz Biotechnology | Cardiomyocyte |