Table 2. RT-qPCR primer sequences used in this study.

Genes	Forward Primer	Reverse Primer	Product Length (bp)	Annealing Temperature (°C)	GenBank Accession No
Tnni3	5′-ACGTGGAAGCAAAAGTCACC-3′	5′-CCTCCTTCTTCACCTGCTTG-3′	201	58	NM_017144.1
Myh6	5′–TGATGACTCCGAGGAGCTTT–3′	5′–TGACACAGACCCTTGAGCAG–3′	234	58	NM_017239.2
Myh7	5′-CCTCGCAATATCAAGGGAAA-3′	5′-TACAGGTGCATCAGCTCCAG-3′	198	58	NM_017240.1
Actc1	5′–CACGGCATTATCACCAACTG–3′	5′–AACAATGCCTGTGGTTCTCC–3′	240	58	NM_019183.1
Gja1	5′-TCCTTGGTGTCTCTCGCTTT-3′	5′-GAGCAGCCATTGAAGTAGGC-3′	167	58	NM_012567.2
Gata4	5′-TCAAACCAGAAAACGGAAGC-3′	5′-CTGCTGTGCCCATAGTGAGA-3′	192	58	NM_144730.1
Nppa	5′-ATACAGTGCGGTGTCCAACA-3′	5′-CGAGAGCACCTCCATCTCTC-3′	209	58	NM_012612.2
Nppb	5′-GGAAATGGCTCAGAGACAGC-3′	5′-CGATCCGGTCTATCTTCTGC-3′	164	58	NM_031545.1
Arbp	5′–CGACCTGGAAGTCCAACTAC–3′	5′–ATCTGCTGCATCTGCTTG–3′	109	58	NM_022402.1