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Hu proteins bind to the AU-rich elements downstream of HuD exon 6. (A) The sequences of the wild type and mutant RNA oligonucleotides are shown. (B) RNA gel mobility shift analysis. The wild type (lanes 1–7) or mutant (lanes 8–14) RNA was incubated with no protein (lanes 1 and 8), increasing amounts (2, 10 and 50 ng) of GST protein alone (lanes 2–4 and 9–11) or GST-HuC sv4 (lanes 5–7 and 12–14).
