Figure 1.

The tyrS complementation tests with pMjYS, pScYS, pMjYSYR and pScYSYR (A). The mutant cells transformed with these plasmids were inoculated on the LB plates containing 1% d-glucose and 34 µg/ml Cm. The three sectors of each half plate represent dilutions of the cells. An illustration of the genetic modifications and plasmid systems in FT3 and FB3 cells (B). The absence of the E. coli TyrRS activity in the strain FT1 (C). TOP10 and FT1 cells were each transformed with pACamKsupF and were then inoculated on the LB plate with 10 µg/ml Cm.