Figure 3.
Isolation and expression analysis of miR-124 expressing cells from wild-type and mir-124 mutants. (A) Embryo isolation, (B) cell dissociation by chitinase and trypsin treatment, (C) embryonic cell culture, (D) enrichment of GFP+ and GFP- cell by FACS, (E) analysis of mRNA expression by Affymetrix arrays.
