Table 2.

Targeting frequencies and site locations in double-target clones

Clone	Overall targeting frequency	Proportion targeted at each sitea	Partial targeting frequency	Chromosomal location	Nearest RefSeq gene	Location of integration site	Distance to transcription start siteb	Predicted size of hph-hybridizing fragmentc
2	1.15 × 10−3	A: 13/20	7.46 × 10−4	Chr 16: 19,636,191	IQCK	Intron 1	−0.9 kb	4.4 kb
		B: 7/20	4.01 × 10−4	Chr 9: 12,804,291	LOC286343	Intron 1	−39.3 kb	7.1 kb
38	2.10 × 10−3	A: 18/20	1.89 × 10−3	Chr 3: 173,242,513	FNDC3B	Intron 1	−1.5 kb	8.4 kb
		B: 2/20	2.10 × 10−4	Chr 16: 30,576,276	PRR14	Intergenic	−6.5 kb	10.3 kb
49	1.32 × 10−3	A: 16/21	1.00 × 10−3	Chr 5: 153,581,833	GALNT10	Intron 1	−31.3 kb	5.5 kb
		B: 5/21	3.14 × 10−4	Chr 4: 167,577,088	SPOCK3	Intergenic	+314.0 kb	7.5 kb

^aRepresents the number of rescued target plasmids from site A or B over the total analyzed for each clone.

^bPositive and negative distances indicate that the integration site is downstream or upstream, respectively, relative to the nearest RefSeq transcription start site.

^cSee Figure 1C for bands of corresponding sizes on Southern blot.